Proteins are enormous biomolecules and macromolecules that are made up of one or more long chains of amino acids. Proteins play a variety of roles in organisms, including catalyzing metabolic events, DNA replication, reacting to stimuli, giving cells and organisms shape, and moving materials from one place to another. Proteins differ mainly in their amino acid sequence, which is governed by their genes' nucleotide sequence and generally culminates in protein folding into a specific 3D structure that dictates its function.
Proteins can be separated from other biological components using a variety of methods, such as ultracentrifugation, precipitation, electrophoresis, and chromatography; the advent of genetic engineering has opened up a slew of new purification options. Typical techniques for researching protein structure and function include immunohistochemistry, site-directed mutagenesis, X-ray crystallography, nuclear magnetic resonance, and mass spectrometry.
What are proteins, what makes up a protein
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